CIDER Webserver: Classification of Intrinsically Disordered Ensemble Regions


Please click here to be taken to CIDER (external site).

CIDER was last updated in August 2020.


CIDER is a webserver being developed by the Pappu Lab for calculating parameters relating to disordered protein sequences. Specifically, this will calculate various parameters which help translate primary sequence information into better understanding how the unstructured ensemble might behave, as well as annotating your sequence on the diagram of states (from ref [1]), which is shown below.

Phase Diagram
Phase Diagram

Development and citation

CIDER was developed by Alex Holehouse, based on code written by James Ahad. For any questions please contact Alex at

Please cite CIDER and localCIDER as;

A.S. Holehouse, R.K. Das, J.N. Ahad, M.O.G. Richardson, R.V. Pappu (2017) CIDER: Resources to analyze sequence-ensemble relationships of intrinsically disordered proteins. Biophysical Journal, 112: 16-21 Access Article

Also, please make sure to cite the original papers were specific sequence insights were first determined (see the CIDER paper for an appropriate list).

[1] Das & Pappu (2013) Proceedings of the National Academy of Sciences USA. 110: 13392-13397